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@#[摘 要] 目的：探究甘露糖结合凝集素2（LMAN2）在激素受体（HR）阳性乳腺癌组织中的表达水平与乳腺癌患者预后的关系及其对MCF-7 细胞增殖和迁移的影响。方法：通过TCGA、Bc-GenExMiner、GEPIA和Kaplan-Meier Plotter数据库分析LMAN2在乳腺癌组织和正常乳腺组织中的差异性表达及其与患者预后的关系。采用小RNA干扰技术将si-LMAN2#1、si-LMAN2#2及si-NC转染至MCF-7细胞，将过表达LMAN载体（pc-LMAN）及空载体pcDNA3.1阴性对照（pc-NC）转染至MCF-7细胞，实验分为si-LMAN2#1、si-LMAN2#2、si-NC、pc-LMAN2和pc-NC组。通过qPCR和WB实验检测各组细胞中LMAN2 mRNA和蛋白的表达水平，CCK-8、克隆形成、Transwell迁移、WB等实验检测敲低和过表达LMAN 2对MCF-7细胞增殖、克隆形成、迁移及AKT信号通路相关蛋白表达的影响。结果：LMAN2在乳腺癌组织中的表达水平显著高于正常乳腺组织（P<0.001）。HR阳性乳腺癌组织中LMAN2表达水平显著高于HR阴性乳腺癌组织（P<0.001）；LMAN2高表达与HR阳性乳腺癌患者不良预后有关联。敲低LMAN2可显著降低MCF-7细胞的增殖和迁移能力（P<0.01或P<0.001），过表达LMAN2可显著提高MCF-7细胞的增殖和迁移能力（均P<0.001）。敲低LMAN2组MCF-7细胞中PTEN和P21蛋白表达水平均显著升高，p-AKT蛋白表达水平显著降低（均P<0.01）。结论：LMAN2在乳腺癌组织和HR阳性乳腺癌组织中高表达，且与不良预后有关联。LMAN2高表达与MCF-7细胞增殖和迁移有关联，其作用机制可能涉及AKT信号通路。
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@#Objective To evaluate the safety of human purified Vero cell rabies vaccine(PVRV)after exposure in China by Meta-analysis.Methods With rabies,vaccine and safety as key words,a systematic search was performed in PubMed,EMBASE,Cochrane and China National Knowledge Infrastructure(CNKI),supplemented by manual retrieval.A Meta-analysis was performed to analyze the incidence of adverse events of two immunization regimens Zagreb and Essen using Review Manager 5.4 software after literature screening and data extraction according to the inclusion and exclusion criteria.Results A total of 12 studies were included,of which 7 were prospective studies and 5 were retrospective studies.Most included in the studies showed a low risk of bias.The incidence of adverse events in Zagreb regimen was significantly higher than that in Essen regimen[relative risk(RR)= 1.01,95% CI = 0.90 ～ 1.14;I2= 73.00%,P<0.05],but there was a high degree of heterogeneity.The incidence of fever,pain and induration in Zagreb regimen was significantly higher than that in Essen regimen(RR = 1.14,0.92 and 0.86,95% CI = 0.82 ～ 1.60,0.73 ～ 1.14 and 0.29 ～ 2.51;I2= 73.00%,P<0.05],but there was a high degree of heterogeneity.The incidence of fever,pain and induration in Zagreb regimen was significantly higher than that in Essen regimen(RR = 1.14,0.92 and 0.86,95% CI = 0.82 ～ 1.60,0.73 ～ 1.14 and 0.29 ～ 2.51;I2= 81%,65% and 92%,respectively,P<0.01).Conclusion Two regimens of PVRV vaccination after exposure showed good safety.However,when adopting Zagreb regimen,attention should be paid to the physical conditions of children and the elderly with relatively poor immunity to avoid adverse events.
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Abstract Objectives Metastasis is one of the biggest challenges in the management of Esophageal Squamous Cell Carcinoma (ESCC), of which molecular mechanisms remain elusive. The present study aimed to explore the roles and underlying mechanisms of Transmembrane protein 26 (TMEM26) in ESCC. Method TMEM26 expressions in tumorous and adjacent tissues from patients with ESCC and in normal esophageal epithelial and ESCC cell lines were detected by immunostaining and western blotting, respectively. The Epithelial-Mesenchymal Transition (EMT), a critical process during metastasis, was investigated by wound healing and Transwell assays, and EMT-related proteins were examined after the TMEM26 alteration in ESCC cell lines. NF-κB signaling activation and Tight Junction (TJ) protein expression were analyzed by western blotting and immunofluorescence, respectively. In vivo verification was performed on the liver metastatic murine model. Results Compared with non-cancerous esophageal tissues and cells, the TMEM26 expression level was higher in ESCC samples and cell lines, where the plasma membrane localization of TMEM26 was observed. The EMT-related processes of ESCC cells were suppressed by RNAi depletion of TMEM26 but aggravated by TMEM26 overexpression. Mechanistically, TMEM26 promoted NF-κB signaling to accelerate EMT in ESCC cells. The plasma membrane presentation and assembly of TJ proteins were impaired by TMEM26. Conclusion Overall, TMEM26 acts as a critical determinant for EMT in ESCC cells by disrupting TJ formation and promoting NF-κB signaling, which may be a potential therapeutic target for treating metastatic ESCC.
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@#Objective - To establish a new non exposed intratracheal instillation method for establishing a rat silicosis model. Methods ， The specific pathogen free SD rats were randomly divided into control group and experimental group with ten rats in ， each group. Rats in the control group were given 1.0 mL of 0.9% sodium chloride solution and rats in the experimental group - were given 1.0 mL of silica suspension with a mass concentration of 50 g/L adopting to the one time intratracheal instillation ， - ， method and then followed by ventilator assisted ventilation immediately. When the tidal volume stabilized at 2.0 mL the ventilator was removed and the tracheal intubation was pulled out. Five rats in each group were sacrificed after two and four ， - Results weeks after modeling and hematoxylin eosin staining and Masson staining of lung tissue were performed. There was ， ， no death in the two groups of rats during the experiment. After two and four weeks the control group had normal lung structure ， ， ， normal alveolar cavity size no inflammatory cell infiltration thin alveolar wall only a small amount of collagen distribution ， around the lung interstitium and bronchus. At the second week of modeling the alveolar wall of the rats in the experimental ， ， ， group was slightly thickened interstitial lymphocytes and macrophages were infiltrated slight hyperplasia was found and a ， small amount of fibroblasts were visible. At the 4th week of modeling the alveolar wall of the rats in the experimental group was ， ， ， ， significantly thickened fibrous nodules were formed and fibroblasts fibrocytes collagen fibers were significantly increased. Conclusion - The combination of ventilator and non exposed intratracheal instillation method can be used to successfully ， ， . establish a rat silicosis model which is simple safe and effective
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@#Objective - To analyze the effect of using vibration tools on the prevalence of work related musculoskeletal disorders （ ） Methods ， - WMSDs in automobile factory workers. By judgment sampling method front line workers with more than one year of working experience in an automobile factory were selected as the research subjects. Musculoskeletal Disorders Questionnaire was used for investigation. The workers were divided into the control group and the vibration tool group. The propensity score ∶ ， matching method was used to balance the confounding factors of the two groups of workers by 1 1 and 568 people were Results included in each group. The prevalence of WMSDs was compared between the two groups after matching. After ， ， ， ， ， ， matching the prevalence of WMSDs in the shoulder elbow hand/wrist upper back waist hip/buttock and knee of workers in ， （ P ） the vibration tool group was higher than that in the control group and the differences were statistically significant all <0.05 .， The prevalence of WMSDs in different body parts of workers in the vibration tool group ranking from high to low was waist ， ， ， ， ， ， ， ， ， ， neck shoulder hand/wrist upper back knee ankle/foot elbow and hip/buttock with the rate of 74.3% 61.3% 54.2% ， ， ， ， ， （P ） Conclusions 54.0% 50.9% 39.4% 35.2% 31.0% and 27.1% respectively <0.01 . The use of vibration tools can ， ， ， ， ， increase the risk of WMSDs in shoulder elbow hand/wrist upper back waist hip/buttock and knee of automobile factory workers. Corresponding measures should be taken to reduce vibration intensity and reduce contact time to protect workers'
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The chemical constituents were isolated and purified by column chromatography and semi-preparative reversed-phase high performance liquid chromatography with silica gel, MCI and polyamide in order to study the chemical constituents of dried flowers of Osmanthus fragrans var. aurantiacus. Their structures were identified by the physical and chemical properties and one-dimensional nuclear magnetic resonance (1H-, 13C-NMR, DEPT), two-dimensional nuclear magnetic resonance (1H-1H COSY, non-decoupled HSQC, HSQC, HMBC), UV, IR and high resolution mass spectrometry data. One new compound (1) and five known compounds (2-6) were isolated from 95% ethanol extract of dried broccoli. They were identified as (9S)-9-hydroxymengastigm-5-en-4-one-9-O-primeveroside (1), oleanolic acid (2), forsythiaside (3), 2-(4-hydroxyphenethyl)-ethanol-(6-acetyl)-β-D-glucopyranoside (4), salidroside (5), and acteoside (6). Compounds (2-6) were isolated from this plant for the first time.
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Objective To investigate the effect of autophagy on the apoptosis of H 9C2 cardiomyocytes.Methods H9C2 cardiomyocytes were incubated with different concentrations of high glucose and high lipids ( HGHL ) for different time.The surface area of cardiomyocytes was measured after HE staining .The cell viability and apoptotic rate were measured by flow cytometry.Western blot was used to detect the expression levels of LC3Ⅱ, p62, Beclin-1,LAMP2 and cleaved caspase-3.Results After treatment with HGHL, the cells appeared hypertrophy in a concentration-and time-dependent manner , the cell hypertrophy was most obvious under the condition of HGHL(500 μmol/L,36 h)(P<0.001).Cell apoptosis increased in a concentration-and time-dependent manner, the apoptotic rate was nearly 50%under the condition of HGHL ( 500 μmol/L,36 h) ( P<0.001 ) .After treatment with HGHL for 24 h, compared with the control group , the expression of LC3Ⅱ was very significantly increased ( P<0.01) , the expression of Beclin-1 and LAMP 2 were significantly decreased ( P<0.05) , but the expression of p 62 was significantly increased ( P<0.01 ) .Compared with the control group and the intervention group , the expression of cleaved caspase-3 was significantly increased ( P<0.01 ) after 1 h of chloroquine pretreatment .Conclusions HGHL induced H9C2 cardiomyocytes hypertrophy promote H 9C2 cardiomyocytes apoptosis in a concentration-and time-dependent manner .HGHL may inhibit autophagy formation and degradation of H 9C2 cardiomyocytes at the same time , leading to abnormal autophagy accumulation of cardiomyocytes , and promote apoptosis , suggesting that inhibition of autophagy may be an important cause for promote apoptosis .
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Objective To investigate the risk factors of schistosomiasis transmission in Jingmen City. Methods The Onco-melania hupensis snails,the wild animal feces,and infection source were selected as the monitoring objects to carry out the schistosomiasis risk monitoring. I-III levels of risk environments were treated with appropriate measures. Results A total of 52 environments and three water systems were monitored and 1542 snails were dissected but no Schistosoma infected snails were found. Nine fecal samples were collected from the areas with snails,and no eggs of Schistosoma were found. Eighty-nine samples of cattle/sheep faces,and mice and dogs were collected,and three samples of cattle feces were found with Schistosoma eggs. Five environments were assessed as Grade II,and 48 environments were assessed as Grade III,and 2 environments were as-sessed as no risk of schistosomiasis transmission. Conclusions In Jingmen City,the mollusciciding work from May to June could decrease the density of snails and the risk of schistosomiasis transmission efficiently. The schistosome-infected cattle were the main infection source,and therefore,the cattle and snails should be administrated simultaneously.
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This study aims to isolate and identify the bacteria from the surface of Musca domestica adults in different livestock farms.The adults of Musca domestica were collected with sweep nets,then the surface of adult flies were washed with sterile water,and the eluted bacteria cultured with LB culture medium.The DNA of the bacteria isolated was extracted,16SrDNA fragment was amplified through general PCR,and the 16SrDNA sequence was blasted with bacteria sequence in NC-BI.Results showed that the number of Musca domestica adults and the bacteria from their surface had significant different in different livestock farms.The population density of Musca domestica adults had linear correlation with the number of bacteria from Musca domestica adults and the number of bacteria genus.Among the bacteria isolated from Musca domestica adults,Staphylococcus,Klebsiella,Escherichia and Serratia were the most common genus in the livestock farms.Most of the bacteria isolated from livestock farm house flies were opportunistic human bacterial pathogen,and the risk of bacterial pathogens spread to people was existed.The population density of house flies reflected the management level of different livestock farms.The house flies could mechanical transmit opportunistic human bacterial pathogen from livestock residue to human.So the house flies control and the monitoring of bacteria on body surface of house flies carried should be strengthened.
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OBJECTIVE: To evaluate the effects of occupational health intervention measurements on occupational health of pre-employment workers. METHODS: Four hundred and three pre-employment workers from different types of occupations and enterprises of different industries in Urumqi from October 2013 to December 2014 were selected as the study objects.Health education intervention measurements including occupational health knowledge,occupational protection theory and practical operations were carried out through expert lectures, distributing promotional materials, on-site or network consulting and other means,which was performed twice per month for over 10 times. A questionnaire survey was performed before and after the intervention on occupational disease prevention and treatment knowledge,attitude and occupational health education demands among the workers. RESULTS: Among the 403 study subjects,the total awareness rate of occupational disease prevention and treatment knowledge after intervention was higher than that before intervention( 88. 09% vs 49. 63%,P < 0. 01). The total positive rate of occupational disease prevention and treatment attitude was higher than that before the intervention( 96. 03% vs 62. 28%,P < 0. 01). The total demand rate of occupational health education was increased compared to that before the intervention( 84. 62% vs 48. 64%,P < 0. 01). CONCLUSION: The occupational health promotion for pre-employment workers can increase the workers 'awareness of occupational disease knowledge,understand and identify occupational disease hazards,and strengthen their consciousness of self protection. It is one of the effective ways to protect the employees' physical and mental health and avoid occupational hazards.